Table 1. Gene essentiality mapping of phage lambda genome.
| Locus_tag | Gene | function/Protein name [17] | EOP_average | S.D. | This work | Literature |
|---|---|---|---|---|---|---|
| lambdap01 | nu1 | DNA packaging protein | 5.5E-06 | 2.1E-06 | E | E [91] |
| lambdap02 | A | TerL | 1.3E-06 | 4.7E-07 | E | E [92,93] |
| lambdap03 | W | gpW family protein | 3.0E-05 | 1.4E-05 | E | E [93,94] |
| lambdap04 | B | portal | 2.5E-05 | 2.1E-05 | E | E [92,93] |
| lambdap05 | C | S49 family peptidase/capsid component; Viral protease | <2.0E-7 | E | E [93,95] | |
| lambdap06 | nu3 | scaffolding protein | <2.0E-7 | E | E [93,96] | |
| lambdap07 | D | head decoration protein | 6.8E-06 | 2.4E-07 | E | E [93,97] |
| lambdap08 | E | major capsid protein | <6.4E-6 | E | E [93,97] | |
| lambdap09 | Fi | DNA packaging protein FI | 2.0E-05 | 0.0E+00 | E | E [93,98] |
| lambdap10 | Fii | head-tail joining protein | <2.0E-7 | E | E [94] | |
| lambdap11 | Z | tail protein | 2.5E-05 | 7.1E-06 | E | E [55] |
| lambdap12 | U | tail protein | 3.5E-05 | 2.1E-05 | E | E [55] |
| lambdap13 | V | tail protein | 1.7E-05 | 1.9E-05 | E | E [55] |
| lambdap14 | G | minor tail protein G | <6.4E-6 | E | E [99] | |
| lambdap15 | T | tail assembly protein T | 1.2E-05 | 1.2E-05 | E | E [99] |
| lambdap16 | H | tail tape measure protein | 1.0E-06 | 9.4E-07 | E | E [99] |
| lambdap17 | M | tail protein | <2.0E-7 | E | E [99] | |
| lambdap18 | L | minor tail protein L | <2.0E-7 | E | E [99] | |
| lambdap19 | K | tail protein | 8.7E-05 | 1.2E-04 | E | E [99] |
| lambdap20 | I | tail component | 2.6E-05 | 2.7E-05 | E | E [100] |
| lambdap21 | J | host specificity protein J | 1.1E-05 | 1.2E-05 | E | E [92] |
| lambdap26 | lom | Outer membrane beta-barrel protein Lom | 8.8E-01 | 5.3E-01 | NE | NE [17] |
| lambdap27 | stf | protail fiber N-terminal domain containing protein | 3.0E+00 | 2.8E+00 | NE | NE [62] |
| lambdap90 | orf206b | hypothetical protein | 4.3E+00 | 1.1E+00 | NE | NE [62] |
| lambdap28 | tfa | tail fiber protein | 6.3E+00 | 5.3E+00 | NE | NE [62] |
| lambdap29 | orf-194 | tail fiber assembly protein | 3.3E+00 | 1.1E+00 | NE | NE [62] |
| lambdap80 | ea47 | 5.5E+00 | 6.4E+00 | NE | NE [17] | |
| lambdap81 | ea31 | 1.2E+00 | 1.1E+00 | NE | NE [17] | |
| lambdap82 | ea59 | ATP-dependent endonuclease | 1.3E+01 | 1.7E+01 | NE | NE [17] |
| lambdap33 | int | tyrosine-type recombinase/integrase | 2.8E+00 | 3.2E+00 | NE | NE [55] |
| lambdap34 | xis | excisionase | 1.6E+00 | 1.1E+00 | NE | NE [55] |
| lambdap35 | NT | |||||
| lambdap36 | ea8.5 | 1.4E+00 | 1.3E+00 | NE | NE [17] | |
| lambdap83 | ea22 | ead/Ea22-like family protein | 2.2E+00 | 2.3E+00 | NE | NE [17] |
| lambdap37 | orf61 | hypothetical protein | 9.7E-01 | 8.1E-01 | NE | NE [17] |
| lambdap38 | orf63 | DUF1382 family protein | 1.3E+00 | 3.8E-01 | NE | NE [17] |
| lambdap39 | orf60a | DUF1317 domin-containing protein | 2.4E+00 | 2.0E+00 | NE | NE [17] |
| lambdap41 | exo | YqaJ viral recombinase family protein | 6.9E-01 | 4.4E-01 | NE | NE [17] |
| lambdap84 | bet | recombination protein Bet | 2.1E+00 | 2.5E+00 | NE | NE [17,101] |
| lambdap42 | gam | host-nuclease inhibitor protein Gam | 1.4E+00 | 1.3E+00 | NE | NE [17,101] |
| lambdap85 | kil | host cell division inhibitory peptide Kil | 1.0E+00 | 0.0E+00 | NE | NE [102] |
| lambdap86 | cIII | protease FtsH-inhibitory lysogeny factor CIII | 1.1E+00 | 3.1E-01 | NE | NE [103,104] |
| lambdap45 | ea10 | DUF2528 family protein | 1.7E+00 | 4.7E-01 | NE | NE [17] |
| lambdap46 | ral | Restriction inhibitor protein ral | 1.1E+00 | 3.2E-01 | NE | NE [60,61] |
| lambdap47 | orf28 | hypothetical protein | 1.3E+00 | 4.7E-01 | NE | NE [17] |
| lambdap48 | sieB | Superinfection exclusion protein B | 1.5E+00 | 7.1E-01 | NE | NE [105] |
| lambdap49 | N | antitermination protein N | 1.8E-04 | 1.2E-04 | E | E [106,107] |
| lambdap53 | rexB | exclusion protein | 1.4E+00 | 1.3E+00 | NE | NE [17,105] |
| lambdap87 | rexA | exclusion protein | 3.8E+00 | 4.0E+00 | NE | NE [17,105] |
| lambdap88 | cI | lysogenic repressor | 5.3E+00 | 6.6E+00 | NE | NE [103,108,109] |
| lambdap57 | cro | lytic repressor | <7.5E-6 | E | E [110] | |
| cII | NT | NE [103,108,109] | ||||
| lambdap89 | O | replication protein | <7.5E-6 | E | E [111,112] | |
| lambdap61 | P | DNA replication protein | <7.5E-6 | E | E [111,112] | |
| lambdap62 | ren | protein ren | 1.20E-02 | 7.7E-06 | E | NE [113] |
| lambdap63 | ninB | recombination protein NinB | 3.00E-05 | 5.4E-04 | E | NE [17,65] |
| lambdap64 | ninC | phosphoadenosine phosphosulfate reductase family protein | clearing on E-1 | E | NE [17,65] | |
| ninD | NT | NE [17,65] | ||||
| ninE | NT | NE [17,65] | ||||
| lambdap67 | ninF | 4.00E-05 | 4.0E-06 | E | NE [17,65] | |
| lambdap68 | ninG | recombination protein NinG | 8.0E-06 | 1.1E-04 | E | NE |
| ninH | NT | NE [17,65,113] | ||||
| lambdap70 | ninI | serine/threonine phosphatase | 4.0E-06 | 5.8E-06 | E | NE [17,65] |
| lambdap71 | Q | antitermination protein | 1.6E-05 | 6.1E-06 | E | E [65] |
| lambdap73 | orf-64 | hypothetical protein | 1.2E-02 | 9.1E-02 | NE | |
| lambdap74 | S | holin/anti-holin | 1.0E-05 | 4.6E-05 | E | E [114] |
| lambdap92 | S’ | holin/anti-holin | 1.0E-05 | 1.7E-04 | E | E [114] |
| lambdap75 | R | endolysin | 6.0E-02 | 5.1E-02 | NE | E [115] |
| lambdap76 | Rz | I-spanin | 5.0E-04 | 1.5E-01 | E | E [116] |
| Rz1 | NT | E [116] | ||||
| lambdap77 | bor | serum resistance lipoprotein Bor | 7.9E-01 | 3.0E-01 | NE | NE [17] |
| lambdap78 | - | putative envelope protein | 1.9E+00 | 1.3E+00 | NE | NE [17] |
| lambdap79 | - | hypothetical protein | 3.0E-04 | 1.1E-04 | E | NE [17] |
| Assay with pQ plasmid | ||||||
| lambdap62 | ren | protein ren | 1.20E-02 | 1.7E+00 | NE | NE [113] |
| lambdap63 | ninB | recombination protein NinB | 3.00E-01 | 9.1E-01 | NE | NE [17,65] |
| lambdap64 | ninC | 1.00E+00 | 1.5E+00 | NE | NE [17,65] | |
| ninD | NE [17,65] | |||||
| ninE | NE [17,65] | |||||
| lambdap67 | ninF | 0.03 | 1.4E+00 | NE | NE [17,65] | |
| lambdap68 | ninG | recombination protein NinG | 0.2 | 2.1E-01 | NE | NE |
| ninH | NE [17,65,113] | |||||
| lambdap70 | ninI | serine/threonine phosphatase | 1 | 1.5E+00 | NE | NE [17,65] |
E, essential; NE, nonessential; NT, not tested.