TABLE 2.
Effect of rpoS::cm on protease production
| Strain | Plasmid | Protease productiona
|
|
|---|---|---|---|
| Medium | Cells | ||
| 92A1552 (wild type) | None | 1,028.56 ± 58.60 | 9.76 ± 0.29 |
| 92A1552 (rpoS::cm) | None | 9.5 ± 2.1 | 10.29 ± 0.25 |
| pBKS | 7.29 ± 3.78 | 10.60 ± 0.14 | |
| pFY1 | 27.17 ± 7.90 | 11.60 ± 0.25 | |
| pFY7 | 5.37 ± 3.59 | 12.56 ± 0.33 | |
| pFY8 | 173.48 ± 18.45 | 10.63 ± 0.36 | |
| pFY9 | 104.83 ± 13.77 | 11.52 ± 0.28 | |
| pFY10 | 7.95 ± 4.23 | 10.91 ± 0.31 | |
| pWKS130 | 5.17 ± 3.52 | 8.55 ± 0.32 | |
a
Means ± standard deviations from the means (n = 4); determined by measurement of fluorescence (per A600 unit) of BODIPY FL peptides which are generated by hydrolysis of BODIPY FL casein. Supernatants of cultures grown in LB for 24 h and the cell sonicates of them were used for analysis.