TABLE 1.

Characteristics of E. coli strains containing smi promoter-luxCDABE fusions

Strain	Plasmid	Gene fused to luxCDABE	Basal RLUa	SM response ratiob	No. foundc
DPD2081	pDEW213	f415	0.2	1.83 ± 0.14	3
DPD2084	pDEW215	yciG	0.27	1.58 ± 0.22	1
DPD2087	pDEW218	inaA	9.8	1.57 ± 0.40	1
DPD2088	pDEW219	yohF	0.14	1.71 ± 0.12	2
DPD2089	pDEW220	o82/o521	1.4	1.54 ± 0.11	1
DPD2090	pDEW221	osmY	0.08	1.78 ± 0.10	1
DPD2092	pDEW223	o513	5.1	1.35 ± 0.19	1
DPD3501	pDEW301	frvX	13.4	3.09 ± 0.96	1
DPD3505	pDEW305	f253a	0.9	1.52 ± 0.29	2
DPD3507	pDEW307	sohA	62.4	1.20 ± 0.06	1
DPD3509	pDEW309	poxB	1.5	1.72 ± 0.09	1
DPD3512	pDEW312	ldcC	0.5	2.10 ± 0.22	1
DPD2083	pDEW201	None	0.002

^aThe RLU reading from the culture grown in the wells of a microplate, untreated with SM, at the initial time point of the primary screen was recorded as the basal light output. This value was expected to roughly correlate to the amount of transcription initiation at promoters upstream of luxCDABE. 

^bCalculated by dividing the RLU of the SM-treated culture by the RLU of the untreated culture at about 180 min (except the ratio for strain DPD3507, which was calculated at 120 min) after addition of cells to SM in the second screening test. Values are means and standard deviations of the triplicate cultures. 

^cNumber of independent plasmids found with the same fusion of E. coli chromosomal DNA to luxCDABE as in the representative strain listed. In each case when multiple hits were obtained, the joint of lux to the chromosomal segment was identical.