TABLE 2.

Effect of rpoS mutation on smi promoter-luxCDABE fusions^a

Plasmid	Gene fused to luxCDABE	RLU inb:		rpoS de- pendence ratioc
		E. coli MP180 (rpoS+)	E. coli UM122 (rpoS)
pDEW213	f415	0.0033 ± 0.0014	0.0079 ± 0.0070	0.42
pDEW215	yciG	1.11 ± 0.09	0.0059 ± 0.0020	188
pDEW218	inaA	2.44 ± 0.43	2.60 ± 0.55	0.94
pDEW219	yohF	0.455 ± 0.040	0.00888 ± 0.00114	51
pDEW220	o82/0521	3.82 ± 0.86	0.642 ± 0.040	6.0
pDEW221	osmY	0.362 ± 0.073	0.0081 ± 0.0018	45
pDEW223	o513	7.09 ± 1.77	9.61 ± 0.74	0.74
pDEW301	frvX	0.161 ± 0.034	0.146 ± 0.013	1.1
pDEW305	f253a	0.947 ± 0.077	0.0187 ± 0.005	51
pDEW307	sohA	30.0 ± 3.0	24.1 ± 2.5	1.2
pDEW309	poxB	2.38 ± 0.30	0.0241 ± 0.0121	99
pDEW312	ldcC	1.69 ± 0.20	0.148 ± 0.014	11

^aVisual inspection in a dark room of the bioluminescence of hundreds of transformants on petri plates confirmed the quantitative results presented. Either there was a dramatic loss of bioluminescence in the rpoS host or there was no visually discernible difference between two host strains. 

^bCultures of two transformants were grown in LB medium containing 150 μg of ampicillin per ml overnight at 37°C, diluted into LB medium containing 50 μg of ampicillin per ml, and grown to log phase (20 to 24 Klett units) at 37°C. The bioluminescence from two 100-μl aliquots of each of the duplicate cultures was measured. Values are means and standard deviations of the four measurements. 

^cCalculated as follows: RLU [transformants of MP180 (rpoS⁺)]/RLU [transformants of UM122 (rpoS)].