TABLE 3.
Effects of 10 mM salicylate on bioluminescence induction responses in mar+ rob+ and Δmar rob hosts
| Plasmid | Gene fused to luxCDABE | Salicylate response ratioa in:
|
|
|---|---|---|---|
| GC4468 (mar+ rob+) | N8452 (Δmar rob) | ||
| pDEW213 | f415 | NDb | ND |
| pDEW215 | yciG | 3.4 | 5.3 |
| pDEW218 | inaA | 20.6 | 0.92 |
| pDEW219 | yohF | 5.4 | 5.2 |
| pDEW220 | o82/o521 | 1.8 | 4.0 |
| pDEW221 | osmY | 2.7 | 3.9 |
| pDEW223 | o513 | 0.05 | 0.21 |
| pDEW301 | frvX | 0.62 | 0.45 |
| pDEW305 | f253a | 2.6 | 1.8 |
| pDEW307 | sohA | 1.1 | 1.0 |
| pDEW309 | poxB | 12.8 | 4.3 |
| pDEW312 | ldcC | 3.8 | 3.2 |
The cultures were grown to log phase (30 to 42 Klett units) in LB medium containing 50 μg of ampicillin per ml. The culture was then split, and 50 μl was added to 50 μl of LB medium containing or lacking 20 mM sodium salicylate, yielding a final concentration of 10 or 0 mM. The response ratio, which was the RLU of the salicylate-treated culture divided by the RLU of the untreated culture, was calculated from the mean of duplicate cultures at 30 min after combining the cells and salicylate.
ND, not determined. The bioluminescence from E. coli GC4468 or N8452 containing pDEW213 was very low and was reduced below the detection limit by addition of 10 mM salicylate.