FIG. 1.
PrPSc deposition in the spleen does not activate local macrophages. PrPSc immunolabeling was performed with the spleen white pulp from NBH-treated (a) and ME7-injected (d) animals at 19 weeks postinjection. White pulp macrophages in healthy (b) and ME7-infected (e) spleens were stained with FA11. Staining for follicular dendritic cells in white pulp germinal centers of healthy (c) and ME7-injected animals (f) was done with 4C11. Metallophil macrophages were stained with antibodies against sialoadhesin (3D6) (g and j) and MOMA-1 (i and l). Staining with ED31 for the scavenger receptor MARCO was performed with spleens of NBH-treated (h) and ME7-injected (k) animals.