Skip to main content
. 2023 Nov 22;12:e74060. doi: 10.7554/eLife.74060

Figure 2. Cryo-EM analyses of residue 417 in regulating the conformation of recombinant SARS-CoV-2 spike ectodomain.

Prototypic SARS-CoV-2 spike ectodomains containing either Lys417 (as in prototypic SARS-CoV-2) or Val417 (as in SARS-CoV-1) were subjected to cryo-EM analyses and the particle distributions for open and closed conformations were calculated. Cryo-EM densities (top), atomic models (middle), and schematic presentations (bottom) for each of the protein classes are shown. (A) K417-spike in the closed conformation. (B) K417-spike in the open conformation. (C) V417-spike in the closed conformation. (D) V417-spike in the open conformation.

Figure 2.

Figure 2—figure supplement 1. Flow chart of cryo-EM data processing for prototypic SARS-CoV-2 spike ectodomain containing Lys417 (K417-spike).

Figure 2—figure supplement 1.

Representative raw cryo-EM image and 2D classes are presented. Further 3D refinements using the good particles generated a 3.2 Å map for the closed spike and a 3.9 Å map for the open spike. Angular distribution plots, the final maps, half-map Fourier shell correlation (FSC) curves, and accompanying local resolution illustrations are displayed.

Figure 2—figure supplement 2. Flow chart of cryo-EM data processing for prototypic SARS-CoV-2 spike ectodomain containing Val417 (V417-spike).

Figure 2—figure supplement 2.

Representative raw cryo-EM image and 2D classes are presented. Further 3D refinements using the good particles generated a 3.8 Å map for the closed spike and a 3.9 Å map for the open spike. Angular distribution plots, the final maps, half-map Fourier shell correlation (FSC) curves, and accompanying local resolution illustrations are displayed.