Table 3. Resistance phenotype of C276Y and F227Y single mutants and C276Y/F227Y double mutant compared to expected phenotype under additive epistasis.
Table 3—source data 1. Individual bioreplicates of EC50 values (nM) obtained from dose-response assays.
elife-85023-table3-data1.xlsx (17KB, xlsx)
| DHODH mutations | TCMDC-125334 EC50 (fold change) | DSM265 EC50 (fold change) | IDI-6273 EC50 (fold change) | Genz669178EC50 (fold change) |
|---|---|---|---|---|
| 3D7 A10 | 140±42.6 | 4.5±1.6 | 502±209 | 6.9±2.2 |
| C276Y | 12.40±3.09 (0.089) | 63.9±19.8 (14.2) | 41.7±12.7 (0.083) | 22.0±6.68 (3.2) |
| F227Y | 408±17.6 (2.9) | 998±50.0 (221) | 2210±289 (4.4) | 18.8±3.39 (2.7) |
| C276Y/F227Y (D-T-F3-C1) | 65.6±22.9 (0.46) | 9042±2985 (2009) | 163±36.3 (0.32) | 69.7±14.5 (10.1) |
| Expected fold change for C276Y/F227Y (additive assumption) | 0.26 | 3138 | 0.37 | 8.64 |
EC50 (nM) of each line is reported, with fold change relative to 3D7 A10 in parentheses. The expected fold change of the C276Y double mutant based on an assumption of additive epistasis is reported for comparison.