FIG. 2.

Reactivities of MAbs in ELISA with detergent-solubilized gp120 (A) and with oligomeric SF162 proteins (B). Detergent-solubilized gp120 was reacted with human MAbs 2909, 447 (anti-V3), b12 (anti-CD4bd), 697 (anti-V2), and 1418 (anti-parvovirus B19). (A) The horizontal line represents the cutoff for the assay (mean optical density for MAb 1418 plus 3 standard deviations). Oligomeric SF162 gp140 (O-SF162gp140), V2 deletion oligomeric gp140 (O-DV2gp140), and a mutant of oligomeric SF162gp140 in which the glycosylation site at position 154 was eliminated (25) (O-GM154gp140) were reacted with human MAb 2909 or murine anti-V2 MAb G3.4 (19).