FIG. 2.

Motility phenotypes of JB1A8v. (A) Motility assays were performed with JB580v, JB41v, and JB1A8v in 0.3% soft agar plates incubated at 23°C for 16 h. (B) Western analysis of flagellin preparations probed with monoclonal antibody 15D8 as described in Materials and Methods. (C) Northern analysis of total cellular RNA isolated from mid-log-phase cultures probed with fleB-specific DNA. The data shown are from a single experiment and are representative of numerous experiments performed with similar results.