TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| Y. enterocolitica 8081v | Serogroup O:8; pYV8081 Nalr | R. Martinez |
| Y. enterocolitica JB580v | Derivative of 8081v; R− M+ Nalr | 31 |
| Y. enterocolitica JB41v | JB580v wild-type inv; inv::phoA Nalr Cmr | This study |
| Y. enterocolitica JB1A8v | mTn5Km derivative of JB41v; invasin expression regulatory mutant; Nalr Cmr Kmr | This study |
| Y. enterocolitica JB16v | JB580v with integrated pJB207; same phenotype as JB1A8v; Nalr Cmr | This study |
| E. coli SM10λpir | thi thr leu tonA lacY supE recA::RP4-2-TC::Mu; Kmr | 32, 56 |
| E. coli DH5α | supE44 ΔlacU169φ80dlacZΔM15 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 20 |
| Plasmids | ||
| pINP41 | pEP184 containing inv Δ412::phoA Cmr | 43 |
| pmTn5Km | mTn5Km on delivery plasmid pUT; Kmr | 14 |
| pTM100 | Mobilizable derivative of pACYC184; Tetr | 38 |
| pCR2.1 | PCR product cloning vector; Ampr Kmr | Invitrogen |
| pEP185.2 | Mobilizable suicide vector; Cmr | 31 |
| pRK2013 | ColE1 Tra (RK2)+; Kmr | 16 |
| pUC19 | Cloning vector; Apr | NEB |
| pJB15 | pUC19 containing 6.0-kb mTn5Km transposon-chromosome junction in SacI site; Apr | This study |
| pJB16 | pUC19 containing 28-kb mTn5Km transposon-chromosome junction in XbaI site; Apr Kmr | This study |
| pKVS2 | pBluescript containing 2.2-kb SacII fragment of 3′ end of fleA, all of fleB, and 5′ of fleC; Apr | 28 |
| pJB207 | pEP185.2 containing DNA derived from pJB16 used to disrupt sspA locus in JB16v; Cmr | This study |
| pJB309 | pTM100 containing E. coli sspA; Tetr | This study |