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. Author manuscript; available in PMC: 2023 Dec 4.
Published in final edited form as: Cell Rep. 2023 Sep 1;42(9):113061. doi: 10.1016/j.celrep.2023.113061

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Anti-Digoxigenin, polyclonal antibody, immunoglobulin Sigma Aldrich Cat# 11333089001; RRID: AB_514496
Bacterial and virus strains
BL21(DE3) New England Biolabs C2527H
ElectroMAX™ DH5α-E Competent Cells Invitrogen 11319019
Chemicals, peptides, and recombinant proteins
MfssrA peptide for sortase ligation
Glass Binding Peptide CGGRSGRRRSHHHRL		 21st Century Biochemicals Synthesized using Fmoc technique and in Apex 396 solid-phase peptide synthesizer N/A
N/A
HaloTag Succinimidyl Ester Ligand O4 Promega P6751
Sulfosuccinimidyl 4-[N-maleimidomethyl] cyclohexane-1-carboxylate Thermo Scientific PI22622
Glucose Oxidase, Aspergillus niger, Recombinant EMD Millipore 345386
Catalase from bovine liver Sigma Aldrich C30
Bovine serum albumin New England Biolabs B9000S
mPEG-Silane, MW 5000 Laysan Bio MPEG-SIL-5000
Biotin-PEG-Silane, MW 5000 Laysan Bio Biotin-PEG-SIL-5K
EDC (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride) crosslinker Thermo Scientific 22980
Streptavidin EMD Millipore 189730
Oligonucleotides
M13MP18 double stranded DNA Bayou Biolabs P-105
5BiosG/AATCCGCTTTGCTTCTGAC IDTDNA, USA N/A
5AmMC6/TTGAAATACCGACCGTGTG IDTDNA, USA N/A
5DigN/TATTGCGTTTCCTCGGTTTC IDTDNA, USA N/A
5AmMC12/TTGAAATACCGACCGTGTGA IDTDNA, USA N/A
Recombinant DNA
pBAD33 plasmid containing MfLon Gur and Sauer, 2008b9 N/A
pFN18A plasmid containing Halo-V13P3-LPETGG-His6 This study, derived from Cordova et al., 201425 N/A
pFN18A plasmid containing Halo-V13P4-LPETGG-His6 This study, derived from Cordova et al., 201425 N/A
pFN18A plasmid containing Halo-V15P4-LPETGG-His6 This study, derived from Cordova et al., 201425 N/A
pFN18A plasmid containing Halo-WT4-LPETGG-His6 This study, derived from Cordova et al., 201425 N/A
pFN18A plasmid containing Halo-WT4.125-LPETGG-His6 This study, derived from Cordova et al., 201425 N/A
pET28b plasmid containing GFPmut3-LPETGG-His6 This study N/A
pET28b plasmid containing cp7-SFGFP-LPETGG-His6 This study, derived from Nager et al., 201121 N/A
pET23b plasmid containing SUMO-V13P-LPETGG-His6 This study N/A
pET23b plasmid containing SUMO-V15P-LPETGG-His6 This study N/A
pET23b plasmid containing SUMO-WT-LPETGG-His6 This study N/A
pET23b plasmid containing SUMO-WT1.125-LPETGG-His6 This study N/A
Software and algorithms
Step finding algorithm Kerssemakers et al., 200631 https://doi.org/10.1038/nature04928
XLSTAT Addinsoft 2022 https://www.xlstat.com/en/solutions/basic
Other
1.25 μm streptavidin coated polystyrene beads Spherotech SVP-10–5 1%w/v, 1.25
1.09 μm streptavidin coated polystyrene beads Spherotech SVP-10–5 1%w/v, 1.09
1.09 μm carboxylate polystyrene beads Polysciences, Inc. 08226