TABLE 1.
Sensitivities of WT and mutant pseudoviruses against a panel of inhibitors
| Pseudovirus | Sensitivitya to:
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| N44*
|
Five-helix inhibitor
|
C34
|
T20
|
sCD4
|
||||||
| GM (nM) | Ratio (P) | GM (nM) | Ratio (P) | GM (nM) | Ratio (P) | GM (nM) | Ratio (P) | GM (nM) | Ratio (P) | |
| JR-CSF | ||||||||||
| WT | 696 | 1.00 | 131 | 1.00 | 198 | 1.00 | 969 | 1.00 | 23.33 | 1.00 |
| NC | 1,430 | 2.05 (<0.001) | 696 | 5.31 (<0.001) | 324 | 1.63 (0.009) | 1,895 | 1.96 (0.003) | 7.12 | 0.31 (<0.001) |
| N | 897 | 1.29 (0.06) | 276 | 2.11 (<0.001) | 382 | 1.93 (<0.001) | 1,047 | 1.08 (0.9) | 8.34 | 0.36 (<0.001) |
| C | 1,184 | 1.7 (<0.001) | 275 | 2.10 (<0.001) | 311 | 1.57 (0.02) | 1,440 | 1.49 (0.087) | 30.69 | 1.32 (0.3) |
| HXB2 | ||||||||||
| WT | 421 | 1.00 | NDb | 8.95 | 1.00 | ND | 0.062 | 1.00 | ||
| NC | 924 | 2.19 (<0.001) | ND | 25.12 | 2.81 (<0.001) | ND | 0.104 | 1.68 (0.006) | ||
| N | 577 | 1.37 (0.01) | ND | 15.71 | 1.76 (0.02) | ND | 0.101 | 1.63 (0.009) | ||
| C | 668 | 1.58 (<0.001) | ND | 14.46 | 1.62 (0.047) | ND | 0.058 | 0.93 (0.9) | ||
a
The GM of the 50% inhibitor concentration was calculated for each inhibitor and pseudovirus. The ratio is fold change of the mutant value compared to the WT value, calculated by dividing the GM for each mutant by the GM for the WT. Values shown represent averages from at least five independent experiments. The N44*, C34, and T20 peptides are shown in Figure 1. The five-helix inhibitor is described in Results. Statistically significant changes are in boldface.
b
ND, not done.