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VP40 promotes tubulin polymerization. The tubulin polymerization assay (see Materials and Methods) was performed in the presence of VP40 or bovine serum albumin (BSA), and polymerization was monitored by light scattering at 340 nm. All data points represent the light scattering of each sample corrected for background (starting point) levels of the same sample. The VP40-only control contains VP40 but no tubulin. Data were recorded for 2 h at 37°C at 3-min intervals. OD, optical density.
