FIG. 1.

Nuclear localization of VP22 (A) but not VP13/14 (B) during infection correlates with HSV-1-induced microtubule reorganization. Vero cells were synchronously infected with HSV-1(F) or mock infected, fixed for indirect immunofluorescence at the times indicated, and double stained with anti-α-tubulin monoclonal (A and B), anti-VP22 polyclonal (A), or anti-VP13/14 polyclonal (B) antibody as described in Materials and Methods. α-Tubulin was visualized with Texas red, and VP22 and VP13/14 were visualized with FITC. Arrowheads in Fig. 1A and B, panels 1 to 3, mark representative cells with detected perinuclear-juxtanuclear MTOCs. Arrowheads in the α-tubulin portion of Fig. 1A, panels 7 to 9, and Fig. 1B, panels 4 to 6, mark cells in which microtubule reorganization occurred during productive HSV-1(F) infection. Thus, in all mock-infected cells and in infected cells at 5 hpi, arrowheads mark cells that have not lost their MTOCs. Also in HSV-1(F)-infected cells, arrowheads correspond to the locations of the VP22 and VP13/14 proteins (Fig. 1A, panels 10 to 12, and Fig. 1B, panels 7 to 9). Merged images (overlay) are also shown.