FIG. 5.

Nuclear localization of VP13/14 and vhs during HSV-1(RF177) infection. Vero cells were synchronously infected with HSV-1(RF177) or mock infected, fixed at the indicated times, and analyzed with indirect immunofluorescence by double-staining using anti-α-tubulin monoclonal and anti-VP13/14 or vhs polyclonal antibodies as described in Materials and Methods. α-tubulin was visualized with Texas Red. VP13/14 and vhs were visualized with AMCA. Arrowheads in α-tubulin panels point to representative cells either with an intact MTOC or cells whose MTOCs have been lost. Thus, in all mock-infected cells and in infected cells at 5 hpi, arrowheads mark cells that have not lost their MTOCs. “Δ212” indicates that RF177 synthesizes a truncated form of VP22.