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. 2005 Apr;79(8):5090–5104. doi: 10.1128/JVI.79.8.5090-5104.2005

FIG. 4.

FIG. 4.

Ad thermostability. A total of 400 VP/cell of Ad5-CMV-GFP, Ad5/11-CMV-GFP, or Ad11-CMV-GFP was incubated at 37 or 48°C for 0, 5, 15, or 30 min in 0.5 ml of medium. After incubation virus was used to infect 293-Ad11-E1B55K cells in 24-well plates, and the number of GFP-positive cells was analyzed by flow cytometry 48 h after infection (n = 3). The relative infection level was normalized to the level of transduction seen with each virus at 0 min. A Student's t test gave the following P values: Ad11 (37°C, 5 min) versus Ad11 (48°C, 5 min), P > 0.3; Ad5 (37°C, 15 min) versus Ad5 (48°C, 15 min), P = 0.0422; Ad5 (48°C, 15 min) versus Ad11 (48°C, 15 min), P > 0.0221; Ad5/11 (48°C, 15 min) versus Ad11 (48°C, 15 min), P > 0.0136; Ad5 (48°C, 30 min) versus Ad11 (48°C, 30 min), P > 0.0012; Ad5/11 (48°C, 30 min) versus Ad11 (48°C, 30 min), P > 0.0045.