CD46-dependent infection of group B fiber targeted Ad vectors. CHO-pcDNA, CHO-CAR, or CHO-C2 cells were preincubated with Ad5, Ad11p, or Ad35 fiber knobs (10 μg/ml) and then infected with Ad5-CMV-GFP, Ad5/11-CMV-GFP, Ad11-CMV-GFP, or Ad5/35-CMV-GFP at an MOI of 500 VP/cell. The following day cell transduction was assessed by flow cytometry of GFP expression.