TABLE 1.
Distinct peptides identified by mass spectrometry of KSHV virions
| Gene product | Accession no. | Size (aa) | Distinct peptides identified |
|---|---|---|---|
| ORF8 (gB) | AAC83368 | 845 | 470-481/DGINQVLEELSR |
| 482-485/AWCR | |||
| 606-611/DYAYLR | |||
| 650-662/LASSVFDLETMFR | |||
| 663-670/EYNYYTHR | |||
| 692-705/DLSEIVADLGGIGK | |||
| 775-884/APPSGGAPTR | |||
| 775-888/APPSGGAPTREEIK | |||
| 789-802/NILLGMMQLQQEER | |||
| 811-818/STPSVFQR | |||
| ORF21 (TK) | gil18845987NP_572073 | 580 | 90-97/FPPRPLIR |
| 126-132/FAFQSPR | |||
| 195-205/LROGGFAFSPR | |||
| 197-205/DGGFAFSPR | |||
| 244-251/TPVTOYR | |||
| 267-281/STLVNAVGCILPQER | |||
| 282-294/VTSFPEPMVYWTR | |||
| 295-301/AFTDCYK | |||
| 328-333/FSLPFR | |||
| 334-341/TNATAILR | |||
| 396-414/ATEGOVVAILTLSSAESLR | |||
| 422-433/KNOGTVEQNYIR | |||
| 423-433/NOGTVEQNYIR | |||
| 547-557/QILSNPAIKPR | |||
| ORF22 (gH) | gil1136819AABO8395 | 730 | 234-245/GHATYDELTFAR |
| 341-349/QYAELFLR | |||
| 391-405/SSQETVLAMVQLGAR | |||
| 487-498/FSKPOSLNIYR | |||
| 498-507/AFSPCFLGLA | |||
| ORF24 | gil10140946 | 752 | 155-166/VSWTLMQIISR |
| 331-339/TALCMAVAK | |||
| 331-343/TALCMAVAKHICR | |||
| 355-367/QQLSLARALVANFEK | |||
| 446-453/LVNNFVIK | |||
| 566-573/IHGSOLTK | |||
| 666-672/VCNSIPK | |||
| 698-706/GAPHMINTK | |||
| 721-729/SYHFAGCAK | |||
| 721-731/SYHFAGCAKAK | |||
| ORF25 (MCP) | gil2246481AAB62606 | 1,376 | 95-106/ISMPTIAHGDGR |
| 158-172/TTTSALQFGMOALER | |||
| 185-193/HAPPVFILK | |||
| 194-203/TLGOPVYSER | |||
| 260-284/GVSTYTTASGQQVAGVLETTDSVMR | |||
| 286-309/LMNLLGQVESAMSGPAAYASYVVR | |||
| 310-321/GANLVTAVSYGR | |||
| 325-332/NFEQFMAR | |||
| 333-347/IVDHPNALPSVEGDK | |||
| 348-359/AALAOGHDEIQR | |||
| 360-381/IDGKFVAIESLQR | |||
| 373-381/FVAIESLQR | |||
| 413-420/YSTSVSVR | |||
| 421-437/GVESPAIQSTETWVVNK | |||
| 458-484/NHNPTQSAQALNQALNQAFPDPDGGHGYGLR | |||
| 485-495/YEQTPNMNLFR | |||
| 496-504/TFHQYYMGK | |||
| 516-530/ALVTTEDLLHPTSHR | |||
| 560-572/TMVGNIPQPLAPR | |||
| 648-658/LAFVNSYHMVR | |||
| 672-679/EAHGHYRK | |||
| 694-702/LAGHETVGR | |||
| 742-753/IGDQNDNPQNR | |||
| 754-760/ATFINLR | |||
| 761-777/GRMEDLVNHVNIYQTR | |||
| 763-777/MEDLVNNVNIYQTR | |||
| 861-876/DILQAGDIRPTVOMIR | |||
| 898-911/RDPAQSFATHEYGK | |||
| 912-930/DVAQTVLVNGFGAFAVADR | |||
| 968-972/LPNQR | |||
| 1030-1042/HRMHPGFAMTVVR | |||
| 1032-1042/MHPGFAMTVVR | |||
| 1043-1056/TDEVLAEHILYCSR | |||
| 1057-1070/ASTSMFVGLPSVVR | |||
| 1057-1071/ASTSMFVGLPSVVRR | |||
| 1138-1150/MKAGVQTGSPGNR | |||
| 1140-1150/AGVQTGSPGNR | |||
| 1151-1162/MOHVGYTAGVPR | |||
| 1201-1217/AACVVSCDAYSNESAER | |||
| 1218-1232/LLTDHSIPOPAYECR | |||
| 1233-1242/STNNPWASQR | |||
| 1243-1255/GSLGDVLYNITFR | |||
| 1268-1277/QFFHKEDIMR | |||
| 1281-1292/GLYTVNEYSAR | |||
| 1346-1365/QTHAPVHMGQYLJEEVAPMK | |||
| ORF26 (TRI-2) | gil11086047A55371 | 305 | 6-14/SIVVNFTSR |
| 15-26/LFADELAALQSK | |||
| 27-38/IGSVLPLGDCHR | |||
| 39-53/LQNIQALGLGCVCSR | |||
| 69-83/CTAVLEEVRPDSLR | |||
| 225-231/LLTALVR | |||
| 323-251/HDRHPLTEVFEGVVPDEVTR | |||
| 235-251/HPITEVFEGVVPDEVTR | |||
| ORF33 | gil2246501AAB62626 | 334 | 10-14/NFLNX |
| 117-126/FPYIAPPPSR | |||
| 135-145/QELVHTSQVVR | |||
| 197-203/VAALLTR | |||
| 217-203/GHVNVFR | |||
| 257-270/NFLGLLFDPIVQSR | |||
| 276-303/ITSHPIPTHVENVLTGVLDDGTLVPSSK | |||
| 304-310/APWVLLR | |||
| 318-324/LLIYECK | |||
| ORF62 (TR1-1) | gil16846032NP_572118 | 331 | 14-25/QVLGLLPPPTHR |
| 45-61/YAASTRPTVGSLHEALR | |||
| 235-250/VNQRPSMALTFFQSGK | |||
| 251-260/GFAEVVAMIK | |||
| 261-268/DHFTDVIR | |||
| 271-275/YIQLR | |||
| 276-282/HELYINA | |||
| ORF63 | gil18846033NP_572119 | 928 | 26-33/LLMEFQLR |
| 69-74/VFALVR | |||
| 75-92/AAYFLEPPSSIOPLEAAR | |||
| 129-141/LLAHYADQIAGFK | |||
| 219-231/TLVTEHHELFVSR | |||
| 248-253/ALAIYR | |||
| 306-312/LESFLSR | |||
| 430-439/HPGISDIPLR | |||
| 445-460/ALAFFVPPAPINTLQR | |||
| 461-471/VYAALPSQLMR | |||
| 480-492/TTWGGAVPANLAR | |||
| 523-534/VGDTEYDEDIVR | |||
| 535-544/SPLFADAFTK | |||
| 562-569/NRALFQIR | |||
| 564-569/ALFQIR | |||
| 592-603/AYFHIMDILEER | |||
| 683-695/LEFEYDSEGDFVR | |||
| 715-724/TIQTTEQATR | |||
| 725-743/EATVAMTTIAKPIYPAYIR | |||
| 748-753/LEYLNR | |||
| 754-760/LNHHILR | |||
| 761-781/IPFPQDALSELQETYLAAFAR | |||
| 800-817/YFGVLFQHQLVPTAIVKK | |||
| 818-844/LLHIDEAXDTTEAFLQSLAQPVVQGQR | |||
| 826-844/DTTEAFLQSLAQPVVQGQR | |||
| 865-882/INPQFTDAQANIPPSIKA | |||
| 888-902/YDVPEVSVDWETYSR | |||
| 903-913/SAFEAPDDELR | |||
| 914-923/FVPLTLAGLR | |||
| ORF75 | gil5669897AAD46504 | 1,296 | 63-84/DVEIQTVLAVLSPLLGYPHVIR |
| 120-128/ELGLQEWAR | |||
| 139-153/ITQTLLEPHPPQFIR | |||
| 216-222/YFVIPGR | |||
| 235-252/QAFGIHGAYTHVHSSVQR | |||
| 256-289/GLGNLLFHSTLFPGGQTQGALTGLYATE | |||
| 301-321/GVQQAEMLQGAGVPTLGGFLK | |||
| 365-379/FEPTOGPTYPNLYR | |||
| 401-409/GPIFSGLNR | |||
| 419-426/HLQALAPR | |||
| 510-517/TSEQPGIR | |||
| 518-528/IVDDLTGETTR | |||
| 529-551/VFSVDQPSSTPPSPWLALSDGVR | |||
| 552-575/VSGHPEDVDWGLFATGSTIHLQLLR | |||
| 655-667/ALPQELLPVPAWR | |||
| 741-760/ELGVALSISSAASSPTLSER | |||
| 792-799/YRVTPDVK | |||
| 991-999/LEHHLGSLR | |||
| 1009-1017/LFSCPTSPR | |||
| 1055-1069/GQSLSGFSGLITCLR | |||
| 1127-1141/SESSPYTYGPTPPQR | |||
| 1157-1161/SVFLR | |||
| 1193-1203/YEQDALEYILR | |||
| 1204-1224/QRGEITLTYHGNAADETLPAR | |||
| 1229-1245/NPTGNSTVAGLTSSDGR | |||
| Actin (beta) | gil14250401AAH08633 | 368 | 12-21/AGFAGDDAPR |
| 22-32/AVFPSIVGRPR | |||
| 89-106/VAPEEHPVLLTEAPLNPK | |||
| 171-176/LALAGR | |||
| 185-189/ILTER | |||
| 190-199/GYSFTTTAER | |||
| 232-247/SYELPDGQVITIGNER | |||
| 278-283/COVDIR | |||
| 322-328/IIAPPER | |||
| 322-329/IIAPPERK | |||
| 353-365/QEYDESGPSIVHR | |||
| α-Enolase | gil4503571NP_001419 | 434 | 1-9/MSILKIHAR |
| 10-15/EIFFDSR | |||
| 16-28/GNPTVEVDLFTSK | |||
| 33-50/AAVPSGASTGIYEALELT | |||
| 82-92/LNVTEQEKIDK | |||
| 93-103/LMIEMDGTENK | |||
| 127-132/GVPLVR | |||
| 163-179/LAMQEFMILPVGAANFR | |||
| 184-193/IGAEVYHNLK | |||
| 203-221/DATNVGDEGGFAPNILENK | |||
| 240-253/VVIGMDVAASEFFR | |||
| 270-281/YISPDQLADLYK | |||
| 307-326/FTASAGIQVVGDDLTVTNPK | |||
| 407-412/YNQLLR | |||
| EF-2B | AAH24689 | 517 | 51-59/GPLMMYISK |
| 69-74/FYAFGR | |||
| 75-85/VFSGLVSTGLK | |||
| 98-108/KEDLYLKPIQR | |||
| 99-108/EDLYLKPIQR | |||
| 158-165/FSVSPVVR | |||
| 179-185/LVEGLKA | |||
| 219-231/DLEEDHACIPIKK | |||
| 265-284/ARPFDPGLAEDIDKGEVSAR | |||
| 307-335/KIWCFGPOGTGPNILTDITK | |||
| 327-335/GVQYLNEIK | |||
| 376-385/GGGQIIPTAR | |||
| 427-444/GHVFEESQVAGTPMFVVK | |||
| 445-460/AYLPVNESFGFTADLR | |||
| 505-517/EGIPALDNFLDKL | |||
| Ezrin (villin 2) | gil21614499NP_003370 | 586 | 152-162/LIPQRVMDQHK |
| 238-246/IGFPWSEIR | |||
| 263-273/KAPDFVFYAPR | |||
| 264-273/APDFVFYAPR | |||
| 361-371/AERELSEQIQR | |||
| 364-371/ELSEQIQR | |||
| 372-379/ALQLEEER | |||
| 382-393/AQEEAERLEADR | |||
| 413-427/SQEQLAAELAEYTAK | |||
| 428-436/IALLEEARR | |||
| 530-542/QLLTLSSELSQAR | |||
| 578-586/QRIDEFEAL | |||
| Hsc70 | S11456a27077 | 350 | 26-36/VEIIANDQGNR |
| 37-49/TTPSYVAFTDTER | |||
| 138-155/TVTNAVVTVPAYFNDSQR | |||
| 160-171/DAGTIAGLNVLR | |||
| 221-236/STAGDTHLGGEDFDNR | |||
| 300-311/ARFEELNADLFR | |||
| 302-311/FEELNAOLFR | |||
| 424-447/QTQTFTTYSONQPGVLIQVYEGER | |||
| 459-469/FELTGIPPAPR | |||
| Hsp90 | HS9A_HUMANP07900 | 732 | 41-45/EIFLR |
| 74-86/ELHINLIPNKQDR | |||
| 185-200/VILHLKEDQTEYLEER | |||
| 292-298/TKIPWTR | |||
| 327-337/HFSVEGQLEFR | |||
| 338-344/ALLFVPR | |||
| 345-354/RAPFDLFENR | |||
| 346-355/APFDLFENR | |||
| 346-355/APFDLFENRK | |||
| 386-399/GVVDSEDLPLNISR | |||
| 458-463/LSELLR | |||
| 489-498/HIYYITGETX | |||
| 567-575/FENLCKIMK | |||
| 632-646/HLEINPOHSIIETLR | |||
| Moesin | AAH01112 | 329 | 28-35/QLFDQVVR |
| 41-53/EVWFFGLQYQDTK | |||
| 54-60/GFSTWLK | |||
| 54-63/GFSTWLKLNK | |||
| 72-79/KESPLLFK | |||
| 73-79/ESPLLFK | |||
| 84-100/FYPEDVSEELIQDITQR | |||
| 101-107/LFFLQVK | |||
| 181-193/GMLREDAVLEYLK | |||
| 185-193/EDALEYLK | |||
| 238-246/IGFPWSEIR | |||
| 255-262/FVIKPIDK | |||
| 263-273/KAPDFVFYAPR | |||
| 264-273/APDFVFYAPR | |||
| Pyruvate kinase | gil20178296P14618 | 531 | 32-42/LDIDSPPITAR |
| 33-43/LDIDSPPITAR | |||
| 44-56/NTGIICTIGPASR | |||
| 93-115/TATESFASDPILYRPVAVALDTK | |||
| 174-186/IYDOGLISLQVK | |||
| 231-246/FGVEQDVDMFASFIR | |||
| 271-278/IENHEGVR | |||
| 279-294/RFDEILEASDGIMVAR | |||
| 280-294/FDEILEASDGIMVAR | |||
| 295-311/GDLGIEIPAEKVFLAQK | |||
| 368-376/GDYPLEAVR | |||
| 377-383/MQHLIAR | |||
| 384-400/EAEAAIYHLQLFEELPR | |||
| 401-422/LAPITSOPTEATAVGAVEASFK | |||
| 448-455/APILAVTR | |||
| 462-467/QAHLYR | |||
| Tubulin | 1TUBA | 440 | 65-79/AVFVDLEPTVIDEVR |
| 85-96/QLFHPEQLITGK | |||
| 113-121/EIIDLVLDR | |||
| 216-229/NLDIERPTYTNLVPYPR | |||
| 244-264/FDGALNVDLTEFQTNLVPYPR |