FIG. 3.

Virus growth curves and DNA replication of AcBacIE1KO and AcBacWT in Sf9 cells. (A) Sf9 cells were transfected with 2.0 μg of DNA from each virus. Cell culture supernatants were harvested at the indicated time points posttransfection and assayed for the production of infectious virus by plaque assay. Each data point represents the average titer derived from multiple plaque assays. Error bars represent standard errors. (B) Slot blot analysis of AcBacIE0-KO and AcBacWT viral DNA replication. Sf9 cells were transfected with 2.0 μg of DNA from AcBacIE0-KO and AcBacWT. At the designated times posttransfection, cells were harvested and cell lysates were prepared and subjected to slot blot analysis. EcoRI fragment T of AcMNPV was labeled with [³²P]dCTP and used as a hybridization probe. DNA levels were quantified by phosphorimager analysis. Error bars represent standard errors.