FIG. 5.

Viral growth curves and slot blot analysis of viral DNA replication of AcBacWT, AcBacSplice, AcBacIE1, AcBacIE1(rev), AcBacIE0, and AcBacIE0 in Sf9 cells. (A) Sf9 cells were infected at an MOI of 5 for each virus, and supernatants were harvested at the indicated times p.i. and assayed for the production of infectious BV by plaque assay. Each data point represents the average titer derived from multiple assays. Error bars represent standard errors. (B). DNA levels in Sf9 cells infected at an MOI of 5 for each virus. At the designated times p.i., cells were harvested and cell lysates were prepared and subjected to slot blot analysis. EcoRI fragment T of AcMNPV was labeled with [³²P]dCTP and used as a hybridization probe. DNA was quantified by phosphorimager analysis, and each column represents the average from two or three independent infections. Samples have been normalized relative to AcBacWT at 18 hpi. Error bars represent standard errors.