FIG. 6.
Western blot time course analysis of IE0, IE1, GP64, VP39, and polyhedrin synthesis in Sf9 cells infected with AcBacWT, AcBacSplice, AcBacIE1, AcBacIE1(rev), AcBacIE0, or AcBacIE0M→A. Each protein was analyzed with a monoclonal antibody specific to that protein. Sf9 cells were infected at an MOI of 5, and at the designated times p.i., cells were harvested and cell lysates were prepared for Western blot analysis. The locations of the IE0- and IE1-specific bands are indicated on the right.