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Time course analysis of the apoptosis inhibitor P35 by Western blot assay of Sf9 cells infected with AcBacWT, AcBacSplice, AcBacIE1, AcBacIE1(rev), AcBacIE0, or AcBacIE0M→A. P35 was analyzed with a specific monoclonal antibody. Sf9 cells were infected at an MOI of 5, and at the designated times p.i., cells were harvested and cell lysates were prepared for Western blot analysis.
