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Southern blots of virion DNA from cells infected with ORF63 deletion mutants. (A) ROka, ROka63-AatII15-3, and ROka63-AatII30-4 DNAs were digested with BclI and AatII and hybridized to an ORF63 probe. (B) ROka, ROka63-AccI, ROka63-SfoI, and ROka63-KpnI DNA were digested with BclI and AscI, and ROka, ROka63-5M, and ROka63-10M were digested with BclI and PvuII, followed by hybridization to an ORF63 probe. Numbers correspond to sizes of DNAs in kilobase pairs.
