TABLE 1.
Bacterial strains, plasmids, and phages
| Strain, plasmid, or phage | Relevant characteristics | Reference or source |
|---|---|---|
| E. coli DH10B | F−mcrA Δ(mrr hsdRMS-mcrBC) φ80dlacZΔM15ΔlacX74 deoR recA1 endA1 | Gibco-BRL |
| Streptomyces strains | ||
| S. hygroscopicus NRRL 5491 | Wild type; produces rapamycin | ATCCa |
| S. hygroscopicus LEK111 | rapL gene disrupted | This study |
| S. lividans JI1326 | Host for phage infection | 7 |
| Plasmids | ||
| pUC18 | Cloning vector in E. coli | Sigma Chemical Co. |
| pLK1 | pUC18 with BamHI site removed | This study |
| pLK2 | pLK1 containing a 3-kb EcoRI fragment corresponding to nucleotides 93956 to 96990 of the rap cluster | This study |
| pLK3 | pLK2 with unique BamHI site removed | This study |
| Phages | ||
| KC515 | c+ attP::tsr::vph | 7 |
| ΦRAPL | KC515 containing a 3-kb insert | This study |
a
ATCC, American Type Culture Collection, Rockville, Md.