TABLE 2.

Summary of DC-SIGN/R binding experiments^a

Analyte	Oligomer state	Ligand	Analyte (μM)	Measured KD	Monomer adjusted KD
DC-SIGN R CRD	Monomer	gp120 (Th14-12)	50.0-0.097	2.9	2.9
DC-SIGN R R8	Monomer	gp120 (IIIB)	3500-0.488	3.9	3.9
DC-SIGN R R5	Mono/Tetra	gp120 (IIIB)	1000-0.96	10.4	10.4
DC-SIGN R R1	Tetramer	gp120 (IIIB)	50-0.0097	0.008	0.002
DC-SIGN R R1	Tetramer	gp120 (Th14-12)	0.8-0.025	0.022	0.005
DC-SIGN R R1	Tetramer	gp120 (NL4-3)	0.8-0.025	0.029	0.007
DC-SIGN R R1	Tetramer	gp120(92Ug21-9)	0.8-0.025	0.031	0.008
DC-SIGN CRD	Monomer	gp120 (NL4-3)	500-1.95	50.8	50.8
DC-SIGN R8	Monomer	gp120 (NL4-3)	500-0.488	86.5	86.5
DC-SIGN R1	Tetramer	gp120 (NL4-3)	50-0.97	9	2.3
DC-SIGN R R8	Monomer	ICAM-3-Fc	500-0.488	54	54
DC-SIGN R R5	Mono/Tetra	ICAM-3-Fc	1000-0.96	100-200	100-200
DC-SIGN R R1	Tetramer	ICAM-3-Fc	50.0-0.0097	62	15
DC-SIGN R R1	Tetramer	ICAM-2-Fc	250-0.097	ND	ND
DC-SIGN R R1	Tetramer	ICAM-1-Fc	250-0.097	ND	ND
DC-SIGN CRD	Monomer	ICAM-3-Fc	500-1.95	331	29.1
DC-SIGN R8	Monomer	ICAM-3-Fc	500-0.488	>500	>500
DC-SIGN R1	Tetramer	ICAM-2-Fc	50-6.25	ND	ND
DC-SIGN R1	Tetramer	ICAM-3-Fc	63-3.91	4.6	1.1
DC-SIGN CRD	Monomer	FcγRIII (CD16)	500-0.488	10.5	10.5
DC-SIGN R1	Tetramer	FcγRIII (CD16)	250-0.488	421.5	16.0
DC-SIGN R R1	Tetramer	FcγRIII (CD16)	250-3.9	15.8	3.95
DC-SIGN R R1	Tetramer	FcγRIIb (CD32)	250-0.487	33.5	8.37

^aLigand immobilization levels and concentration range of injected analyte are indicated. For comparison, monomer K_D values were divided by respective receptor oligomerization states. All concentrations and dissociation constants represented as 1 × 10 M⁻⁶ (μM) unless otherwise indicated. Each binding experiment was performed with serial 2× dilutions of analyte and is representative of either multiple single-injection experiments or averaged triplicate injections. ND, nondetectable; Mono/Tetra, monomer-tetramer.