Figure 4.

OTUB2 stabilizes β-Catenin by reducing its poly-ubiquitination. (A) Wild-type and catalytic-site mutated OTUB2 plasmids with a Flag tag were transfected into HCT116 and SW480 cells for 48 hours, followed by western blot analysis. (B and C) Cycloheximide (CHX) chase assay was performed and detected by western blot (B). Optical density was also measured, and statistical analysis was conducted (C). (D) Co-immunoprecipitation (Co-IP) was performed to evaluate the ubiquitination of β-Catenin.