Skip to main content
. 2023 Nov 15;13(11):5382–5393.

Figure 6.

Figure 6

I-BET726 inhibits OTUB2 expression in colorectal cancer cells. (A) The effect of I-BET726 on MYCN-amplified neuroblastoma cell line CHP-212 (GEO profile: GDS5364/ILMN_1799198). (B) The effect of I-BET726 on non-MYCN-amplified neuroblastoma cell line SK-N-SH (GEO profile: GDS5365/ILMN_1799198). (C and D) HCT116 (C) and SW480 (D) cells were incubated with indicated concentrations of I-BET726 for 24 hours, followed by qRT-PCR to detect the mRNA levels of OTUB2. (E) HCT116 cells were incubated with indicated concentrations of I-BET726 for 24 hours, followed by western blot analysis. (F) HCT116 cells overexpressed OTUB2 or β-catenin were incubated with 2 μM I-BET726 or vehicle for 24 hours, followed by CCK-8 assay to detect the cell viability. (G) Schematic model showing that OTUB2 deubiquitinates β-Catenin and mediates β-Catenin signaling in CRC cells, which is abolished by I-BET726 treatment through inhibiting the transcription of OTUB2. *P<0.05, **P<0.01, ***P<0.001.