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. 2023 Nov 15;13(11):5254–5270.

Figure 3.

Figure 3

LX-2 secretes EVs to carry PRDM16 into HCC cells. A. The knockdown efficiency of shRNAs for PRDM16 in LX-2 cells was measured using RT-qPCR and western blot analysis. B. Effect of co-culture with LX-2 cells with or without knockdown of PRDM16 on PRDM16 expression in HCC cells. C. Observation of the morphology of extracted EVs using TEM. D. The particle size of the extracted EVs using NTA. E. The expression of EVs marker proteins using western blot assays. F. PRDM16 mRNA expression in LX-2 and extracted EVs by RT-qPCR. G. Uptake of PKH67-labeled EVs by HCC cells observed under fluorescence microscopy and quantification of the average fluorescence intensity of PKH67. H. The effect of EVs treatment on PRDM16 expression in HCC cells was measured using RT-qPCR. The results represent means ± SD, *P < 0.05 vs sh-NC; #P < 0.05 vs LX-2 cells; &P < 0.05 vs EVs-NC; @P < 0.05 vs PBS. All experiments were repeated at least three times, one-way or two-way ANOVA.