Figure 4.

Encapsulated PRDM16 is responsible for the pro-carcinogenic effect of LX-2-derived EVs. A. The proliferative activity of HCC cells after EVs treatment was measured using CCK8. B. EdU⁺ cells in HCC cells after EVs treatment were measured using EdU staining. C. EMT-related protein expression in HCC cells after EVs treatment was measured using western blot assays. D. The migratory activity of HCC cells after EVs treatment was measured using Transwell assay. E. The invasive activity of HCC cells after EVs treatment was measured using Transwell assay. The results represent means ± SD, *P < 0.05 vs PBS; #P < 0.05 vs EVs-NC. All experiments were repeated at least three times, two-way ANOVA.