Figure 5.

EVs-derived PRDM16 regulates the Notch signaling pathway in HCC cells. A. The KEGG enrichment analysis of genes positively correlated with PRDM16 in HCC. B. Correlation between NOTCH1 expression and PRDM16 expression in HCC. C. The schematic for the Notch signaling pathway. D. The effect of EVs treatment on Notch signaling pathway activity in HCC cells was measured using luciferase reporter assays. E. Effect of EVs treatment on the expression of NOTCH1 and the downstream factors HES1 and HES5 in HCC cells by RT-qPCR. F. The enrichment ability of PRDM16 on the NOTCH1 promoter was measured using ChIP-qPCR. G. The effect of PRDM16 carried by EVs on the transcriptional activity of the NOTCH1 promoter in HCC cells was measured using luciferase reporter assays. The results represent means ± SD, *P < 0.05 vs PBS; #P < 0.05 vs EVs-NC; &P < 0.05 vs IgG. All experiments were repeated at least three times, two-way ANOVA.