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LX-2-derived EVs affect HCC growth in vivo via PRDM16/Notch signaling. (A) The volume of xenografts in mice. (B) Weight of harvested xenografts in mice. Immunohistochemical detection of PRDM16 (C), NOTCH1 (D), HES1 (E), and HES5 (F) expression in xenograft tumors. The results represent means ± SD (n = 8), *P < 0.05 vs PBS; #P < 0.05 vs EVs-NC; &P < 0.05 vs EVs-NC + DMSO. One-way or two-way ANOVA.
