FIG. 2.

DEN-2 virus replication in the presence of five virus-specific antisense P4-PMO compounds and one random-sequence P4-PMO (DScr) at 6 days after infection of Vero cells. Cultures were pretreated with 20 μM P4-PMO for 12 h before infection with DEN-2 virus at an MOI of 2.0. Incubation with 20 μM P4-PMO continued following viral adsorption. The mean log₁₀ viral titers of duplicate wells at day 6 are indicated above each bar. The horizontal line at 2.2 log₁₀ indicates the sensitivity limit in this experiment. Viral replication in the presence of 5′SL and 3′CS was suppressed to undetectable levels in this experiment (open bars). Abbreviated compound names and target viral genomic regions are as indicated in Fig. 1B.