Figure 2.

Disruption of norepinephrine synthesis in Dbh^LC-null and Dbh^flx/flx (Dbh^hypo) mice. (A) Coronal sections from Dbh^wt/wt, Dbh^flx/flx, and Dbh^LC-null mice showing DBH (green) and/or TH (magenta) expression in the LC (representative images from n = 4 female and 4 male mice per genotype). Scale bar = 100 μm. (B) Levels of norepinephrine and dopamine or DOPAC in the hippocampus, adrenal, and plasma of Dbh^wt/wt, Dbh^flx/flx, and Dbh^LC-null mice. Data are mean ± SEM. For hippocampus samples, n = 7 female (closed circles) and 8 male (open circles) Dbh^wt/wt (includes 8 En1^Cre/wt and 7 En1^wt/wt), 5 female and 5 male Dbh^flx/flx, and 11 female and 13 male Dbh^LC-null mice. For adrenal, n = 8 female and 8 male Dbh^wt/wt (8 En1^Cre/wt and 7 En1^wt/wt), 4 female and 4 male Dbh^flx/flx, and 4 female and 4 male Dbh^LC-null mice. For plasma samples, n = 14 female and 18 male Dbh^wt/wt (17 En1^Cre/wt and 15 En1^wt/wt), 10 female and 9 male Dbh^flx/flx, and 10 female and 10 male Dbh^LC-null mice. ∗∗∗∗p < .0001, ^###p = .0004 (main effect of sex). LC, locus coeruleus; TH, tyrosine hydroxylase; wt, wild-type.