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. 2005 Apr;25(8):3087–3108. doi: 10.1128/MCB.25.8.3087-3108.2005

FIG. 2.

FIG. 2.

FIG. 2.

NLSs of Fli-1 can target GFP-Fli-1 fusion protein into the nucleus of UT-7/TPO and K562 cells. (A) Schematic representations of Fli-1 fragments used in the Mk-specific cell lines. The full-length Fli-1-GFP (a), the NLS1-GFP fragment (b), the NLS2-GFP fragment (c), the Fli-1-GFP fragment aa 1 to 75 (d), and the Fli-1-GFP fragment aa 127 to 196 (e) were transfected into the above-mentioned cell lines. CLSM images of K562 and UT-7/TPO are shown in panels B and C, respectively. Lanes 1 show the GFP fusion proteins. Lanes 2 show propidium iodide staining of the nuclei. Lanes 3 show the merge of lanes 1 and 2. Lanes 4 show the transmission images.