Table 2.
Effect of metal ions and EDTA on the XylRc activity
Additive | Relative activity (%) | |
---|---|---|
1.0 mmol L−1 | 5.0 mmol L−1 | |
Control | 100 ± 2.4 | 100 ± 2.4 |
K+ | 128.6 ± 2.9* | 88.6 ± 6.7 |
Ca2+ | 122.9 ± 3.5* | 104.8 ± 6.2 |
Mg2+ | 120.1 ± 5.6* | 103.9 ± 1.8 |
NH4+ | 113.8 ± 6.5* | 100.9 ± 2.3 |
Co2+ | 110.7 ± 6.8 | 91 ± 6.3 |
Li2+ | 107 ± 6.1 | 101 ± 0.2 |
Mn2 | 101.8 ± 7.1 | 112 ± 8.3 |
Sr2+ | 104 ± 4.2 | 87 ± 6.2 |
Zn2+ | 91.1 ± 3.6 | 86.0 ± 3.6 |
Al3+ | 54.9 ± 7,7* | 45 ± 6.2* |
Cu2+ | 100.6 ± 1.7 | 26.1 ± 3,4* |
Fe3+ | 64.4 ± 2.2* | 48.8 ± 4.9* |
Fe2+ | 71.3 ± 9.8* | 0* |
Hg2+ | 0* | 0* |
EDTA | 119.9 ± 3.8* | 86.7 ± 4.5 |
Control Assay without addition of ions was considered as control (100%) corresponding 220 ± 6.0 U mg−1
*Significantly different from control group (p < 0.05)