Skip to main content
. 2023 Dec 4;6:1226. doi: 10.1038/s42003-023-05614-3

Fig. 4. Midgestational periportal hepatocyte proliferation confers glycogen storage capacity to the maternal liver.

Fig. 4

a Representative PAS-stained images of livers from NP and 16 dpc mice injected with AAV8-null or AAV8-p21 at the indicated time points. Mice were fasted for 24 h (fast), followed by intraperitoneal administration of glucose for 2 h (2 h). Scale bars, 100 μm. b Liver glycogen content of 16 dpc mice injected with AAV8-null and AAV8-p21 at the indicated time points (n = 4 mice). Samples were collected 2 h after glucose administration to fasted mice. Intraperitoneal glucose tolerance test (c) and corresponding area under the curve (AUC) (d) of AAV8-null and AAV8-p21 mice at dpc16 (null, n = 6 mice; p21, n = 5 mice). e Placental glycogen content of 16 dpc mice treated as in (b) (n = 4 mice). f Serum glucose concentrations of ad libitum fed mice (null NP → NP, n = 4 mice; p21 NP → NP, n = 4 mice; null NP→dpc16, n = 5 mice; p21 NP→dpc16, n = 4 mice). g Representative images of E16.5 embryos from AAV8-null or AAV8-p21-injected dams. Scale bars, 10 mm. Average embryo mass (h) and average litter size (i) at E16.5 from AAV8-null or AAV8-p21-injected dams (null NP→dpc16, n = 4 dams; p21 NP→dpc16, n = 4 dams; null dpc10→dpc16, n = 4 dams; p21 dpc10→dpc16, n = 4 dams). i Pearson product-moment correlation coefficient between litter size and mean litter weight for i. Data were compared using the two-tailed Studentʹs t test. Data are mean ± s.e.m. (b, d, e, f, h, i).