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. 2023 Dec 4;6:1226. doi: 10.1038/s42003-023-05614-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Venn diagram of laser capture microdissection RNA-seq analysis showing DEGs for NP vs. 8 dpc (left) and NP vs. 16 dpc (right) in each zone. b Heatmap of DEGs for NP vs. 8 dpc in each zone. The mean of three independent experiments is shown. c GO analysis of upregulated genes in the PV zone at 8 dpc. d Representative immunofluorescence images for Hmmr protein and phalloidin in NP, dpc8 and dpc16 mouse liver lobules. PV and CV areas are indicted by white-dotted lines. Scale bars, 100 µm. Percentages of Hmmr⁺ hepatocytes in the PV (e) and CV (f) zones of maternal liver lobules. Each point represents the mean of n = 5 sections/mouse from n = 4 mice. Data were compared using Dunnett’s multiple comparison test (mean ± s.e.m.) (e, f).