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. 2023 Dec 4;6:1226. doi: 10.1038/s42003-023-05614-3

Fig. 6. Hmmr regulates midgestational periportal hepatocyte proliferation and maternal glucose homeostasis.

Fig. 6

a Experimental design for AAV8-shHmmr injection. b Representative immunofluorescence images for Hmmr protein and relative expression of Hmmr mRNA in the livers of AAV8-shHmmr and control AAV8-null injected mice at 8 dpc (n = 4 mice). Scale bars, 100 µm. c Percentages of Ki67+ hepatocytes in the PV zone of livers of AAV8-shHmmr injected mice at 8 dpc (n = 4 mice). d Percentages of Ki67+ hepatocytes in the CV zone of livers of AAV8-shHmmr injected mice at 16 dpc (n = 4 mice). e Liver mass (top graph) and liver-to-body mass ratio (bottom graph) of mice injected with AAV8-null or AAV8-shHmmr at the indicated time points (Null NP→dpc8, n = 4 mice; shHmmr NP→dpc8, n = 5 mice; Null NP→dpc16, n = 9 mice; shHmmr NP→dpc16, n = 4 mice). f Liver glycogen content of 16 dpc mice injected with AAV8-null and AAV8-shHmmr (n = 4 mice). Samples were collected 2 h after glucose administration to fasted mice. Intraperitoneal glucose tolerance test (g) and corresponding AUC (h) of AAV8-null and AAV8-shHmmr mice at dpc16 (null, n = 5 mice; shHmmr, n = 4 mice). i Placental glycogen content of 16 dpc mice treated as in (f) (n = 4 mice). j Representative images of E16.5 embryos from AAV8-null and AAV8-shHmmr-injected dams. Scale bars, 10 mm. Average embryo mass (k) and average litter size (l) at E16.5 from AAV8-null or AAV8-snHmmr-injected dams (n = 4 dams). m Pearson product-moment correlation coefficient between litter size and mean litter weight for (l). Data were compared using the two-tailed Studentʹs t test. Data are mean ± s.e.m. (b–f, h, i, k, l).