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. 2005 Apr;25(8):3324–3337. doi: 10.1128/MCB.25.8.3324-3337.2005

FIG. 10.

FIG. 10.

Effect of wortmannin and U0126 on c-Jun-regulated anchorage-independent growth. (A) Rat1a-c-Jun4 cells were grown in the absence of doxycycline (−cJun), the presence of 2 μg of doxycycline per ml (+cJun), and the presence of doxycycline and 50 nM wortmannin (+cJun + wortmannin). A total of 50 nM wortmannin was sufficient to inhibit phosphorylation of AKT1 and prevent c-Jun-regulated anchorage-independent growth. (B) Cells grown in the absence of doxycycline (−cJun), the presence of 2 μg of doxycycline per ml (+cJun), and the presence of doxycycline and 50 nM U0126 (+cJun + U0126). U0126 at 50 nM prevented phosphorylation of p-ERK1/2 but had little effect on c-Jun-regulated anchorage-independent growth.