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. 2005 Apr;25(8):3324–3337. doi: 10.1128/MCB.25.8.3324-3337.2005

FIG. 2.

FIG. 2.

Ras-GRF1 promoter activation by c-Jun/AP-1. (A) Mouse Ras-GRF1 promoter constructs used in transient transfection assays. Construct 84f spans from −1270 to +1130 of the promoter region and encompasses both of the putative AP-1 binding sites. Construct 84ΔA includes the −489 to +1130 promoter region, and 84ΔBC contains the −1270 to −489 promoter region. All promoter fragments were cloned into pGL2-Basic. (B) Transient transfection on the Ras-GRF1 promoter constructs into Rat1a-c-Jun4 cells grown with (+dox) and without (−dox) doxycyline. (C) 84ΔBC activity in Rat1a-GFP and Rat1a-c-Jun4 cells grown as described for panel B. (D) 84ΔBC activity in the presence of cotransfected c-Jun in the human, rat, and mouse glioblastoma cell lines, T98GBM, G6, and Neuro2a, respectively, compared to activity in Rat1a-c-Jun4 cells. The results show the mean ± standard deviation of triplicate experiments. *, P < 0.05.