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. 2005 Apr;25(8):3324–3337. doi: 10.1128/MCB.25.8.3324-3337.2005

FIG. 3.

FIG. 3.

ChIP assays identify the rat Ras-GRF1 promoter as an AP-1 target gene. (A) The rat Ras-GRF1 promoter equivalent to that of the mouse 84ΔBC region (−1270/−489). Two potential AP-1 binding sites were identified, and primers (F1 and R1 and F2 and R2) flanking these sites were designed for ChIP analysis. (B) ChIP of c-Jun was performed with Rat1a cells with doxycycline-inducible HA-c-Jun. Chromatin from control (−dox) and HA-c-Jun-expressing (+dox) cells was immunoprecipitated with the HA antibody or nonspecific IgG. PCR was performed with primer sets (F1 and R1 and F2 and R2) specific for the rat Ras-GRF1 promoter regions spanning the potential AP-1 binding sites. (C) Real-time PCR analysis using ChIP DNA and primers F1 and R1. The data shown were corrected for nonspecific interactions detected with the IgG control ChIP assay. The results represent the mean ± standard error of five independent ChIP assays. P ≤ 0.05.