FIG. 6.

Elk-1 activation is Ras dependent in c-Jun-overexpressing cells. (A) Rat1a-GFP and Rat1a-c-Jun4 cells were transiently cotransfected with plasmids encoding Gal-Elk-1 fusion protein and the reporter construct 5× Gal-Luciferase (Gal5-Luciferase). The cells were grown nonadherently for 48 h in the absence (−) or presence (+) of 2 μg of doxycyline per ml. Renilla luciferase activity was used to control for transfection efficiencies. Luciferase activities were measured with the Promega dual luciferase assay kit. Data are represented as a fold change relative to controls (−dox). *, P ≤ 0.01 relative to controls (−dox). (B) Transient transfection with a construct containing two AP-1 DNA-binding sequences fused to the luciferase reporter gene (TRE2-Luciferase). The results show the mean ± standard deviation of at least three independent experiments, each performed in triplicate. *, P < 0.05.