FIG. 7.
Dominant-negative RasN17 inhibits c-Jun-regulated activation of Elk-1 activity. (A) Rat1a-c-Jun4 cells were transfected with pCMV-RasN17 or pCMV control vector as described in the legend to Fig. 4. Reporter 5× Gal5-Luciferase (Gal5-Luciferase) activation was corrected for transfection efficiency with Renilla luciferase, and the fold change relative to controls (−dox) is shown. *, P < 0.05 compared to with doxycycline. (B) TRE2-luciferase activity in the presence of pCMV-RasN17 and pCMV vector at the concentrations shown. *, P < 0.05, significantly down-regulated compared to with doxycycline; **, P > 0.05, not significantly different from with doxycycline. Results are representative of the mean ± standard deviation of assays performed in triplicate. (C) Elk-1 activation of the 5× Gal5-Luciferase construct in the presence of doxycycline and sense and antisense Ras-GRF1 oligomers. Ras-GRF1 oligomers were added before transfection and included at a concentration of 10 μM for the duration of the assay. Results represent the mean ± standard deviation of assays performed in triplicate.