FIG. 2.

Tissue-specific and uniform expression of the hAGT gene in three independent TgM lines. (A) Northern blot analysis of hAGT mRNA isolated from several tissues obtained from 1-month-old female (F) animals of three wild-type TgM lines. The kidney was isolated from a male (M) animal as well. The RNA blot was hybridized to hAGT, mouse HNF4α, or β-actin (41) probes. (B to I) In situ hybridization analysis of the hAGT expression in the kidney of wild-type (WT; B to E) and mutant (mutC/J; F to I) hAGT TgM. Kidney sections were hybridized with the hAGT antisense (B, F, D, and H) and sense (C, G, E, and I) probes. The scale divisions are 10 μm.