FIG. 10.
Chromatin immunoprecipitation with MEF-2A-specific antibody. C2C12-DM (in differentiation medium for 72 h) cells were formaldehyde cross-linked and processed for ChIP assays with antibodies (Abs) against MEF-2A (Santa Cruz) or IgG (as the control). PCR products, obtained with primers flanking the MEF-2 binding site of the AβH-J-J P2 promoter or with primers amplifying a region 29 kb upstream of the P2 promoter (negative control), were analyzed by gel electrophoresis. Input, control sample before the immunoprecipitation. M, pUC Mix Marker 8 (Fermentas).