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. 2005 Apr;25(8):3261–3275. doi: 10.1128/MCB.25.8.3261-3275.2005

FIG. 10.

FIG. 10.

Chromatin immunoprecipitation with MEF-2A-specific antibody. C2C12-DM (in differentiation medium for 72 h) cells were formaldehyde cross-linked and processed for ChIP assays with antibodies (Abs) against MEF-2A (Santa Cruz) or IgG (as the control). PCR products, obtained with primers flanking the MEF-2 binding site of the AβH-J-J P2 promoter or with primers amplifying a region 29 kb upstream of the P2 promoter (negative control), were analyzed by gel electrophoresis. Input, control sample before the immunoprecipitation. M, pUC Mix Marker 8 (Fermentas).