FIG. 11.

Effect of HDAC4, CamKI, and calcineurin on P2 promoter activation by MEF-2A. (A) C2C12-DM cells were transfected with the −159 construct in the presence of MEF-2A expression vector (white bars) and the indicated HDAC4 expression constructs (full-length, wild-type HDAC4; Δ catalytic d., HDAC4 lacking the catalytic domain; Δ MEF-2 bind. d., HDAC4 lacking the MEF-2 binding domain). Transient transfection and luciferase assays were performed in triplicate, and the data were normalized to Renilla luciferase activity and are reported as ratios (means ± SD) to the pGL3-basic firefly luciferase (LUC) reporter vector. (B) The same experiment whose results are presented in panel A was performed with the −159 construct, the MEF-2A expression vector, and the indicated CamKI and calcineurin expression constructs. (C) C2C12-GM cells were transfected with the −159 construct alone or in the presence of CaMKI expression vector and the indicated HDAC4 expression constructs.