FIG. 3.

Promoter activity of serial deletion constructs of the AβH-J-J −686 to +115 nucleotide sequence (the P2 transcription initiation site is referred as +1). C2C12 cells were transfected with sequentially deleted reporter constructs (represented in the left side of the figure). Transient transfection and luciferase assays were performed in triplicate, and the data were normalized to Renilla luciferase activity and reported as a ratio to pGL3-basic RLU (means ± SD). pGL3-basic is a firefly luciferase (LUC) reporter vector with basal promoter activity.