FIG. 4.

Promoter activity of serial deletion constructs of the AβH-J-J −686 to +115 nucleotide sequence in soleus muscle of adult rats. Soleus muscles were injected with 50 μg of serial deletion constructs, and the calf was exposed to electrical pulses. At 48 h after transfection each muscle was homogenized in lysis buffer and assayed for luciferase activity. Transient transfection and luciferase assays were performed in triplicate, and the data were normalized to Renilla luciferase activity and reported as a ratio to pGL3-basic RLU (means ± SD). pGL3-basic is a firefly luciferase (LUC) reporter vector with basal promoter activity.