FIG. 6.
Binding of nuclear factors to the AβH-J-J promoter 2. (A and B) Band shift assays performed using J-kBmer (−170 to −151), J-E-box (−177 to −151), and NFkBlgmer and MyoDmer (Table 1) probes and 2 to 3 μg of nuclear extracts (NE) from RD, C2C12-DM-8 h (C2C12 cells were induced to differentiate in DM for 8 h and a nuclear extract was prepared), and H9c2 cells or 0.05 μg of human recombinant p50 and purified human Sp1 transcription factors (Promega), as indicated. (-), probe was incubated with nuclear extracts in the absence of competing oligonucleotides. (C and D) Band shift assays performed using J-GRmer (−241 to −222), J-MEF-3mer (−52 to −32), and MEF-3mer (Table 1) probes and 2 to 3 μg of nuclear extracts (NE) from C2C12-GM and RD cell lines. (-), probe was incubated with nuclear extracts in the absence of competing oligonucleotides. In this figure, competing oligonucleotides (Table 1) were added at a 100-fold molar excess. Arrows indicate the specific complexes.